Global proteomics offers near full proteome coverage in various samples, for instance identifying up to 10,000 proteins in human tissue, such as liver, kidney, or brain. It serves as the method of choice for untargeted discovery in non-hypothesis-driven research, allowing researchers to comprehensively explore and analyze proteins in a sample. Beyond identification, global proteomics is pivotal in initiating biomarker discovery and functional studies. Biomarkers identified in the discovery phase can then be validated in a targeted phase. Its ability to survey the protein landscape provides a starting point for detailed investigations into biological processes, disease mechanisms, and potential therapeutic targets.
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