LC-MS (Liquid Chromatography online-coupled to Mass Spectrometry) offers significant advantages in analytical chemistry and bioanalysis. Its exceptional sensitivity allows for the detection of analytes at low concentrations, which is crucial for biomarker and trace substance identification. Simultaneous measurement of multiple analytes, such as in High-Content Screening, provides comprehensive sample analysis, offering a holistic view of composition. The robust performance of LC-MS enhances data reliability, while its high throughput capabilities enable efficient handling of hundreds of samples across various fields. LC-MS methods also expedite research and decision-making with quicker turnaround times compared to traditional techniques.