The era of middle-throughput

A decade ago, cohorts in LC-MS-based proteomics comprised only a couple of samples. Since then, significant improvements in sample preparation, LC-MS instrumentation, and data analysis and interpretation have transformed the field. These advancements now enable researchers to measure cohorts ranging from hundreds to thousands of samples with an analytical depth that spans thousands of proteins. The evolution of automation has played a crucial role, allowing for error-free processing of large sample numbers swiftly and in parallel. This progress has not only expanded the scope of proteomic research but also accelerated discoveries in biomedical and clinical sciences.